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REVIEW
Year : 2017  |  Volume : 6  |  Issue : 3  |  Page : 136-139

Detection of bacterial biofilm in uterine of repeat breeder dairy cows


1 Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
2 Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
3 Department of Pathology, School of Veterinary Medicine, Shahrekord University, Shahrkord, Iran
4 Shiraz Molecular Research Center, Dr Daneshbod Lab, Shiraz, Iran
5 Departments of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

Correspondence Address:
Mohammad Rahim Ahmadi
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz
Iran
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Source of Support: None, Conflict of Interest: None


DOI: 10.12980/apjr.6.20170308

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Objective: To determine the possibility of presence of bacterial biofilm in the uterus of repeat breeder cows and to evaluate the effect of mucolytic agent in cleanup of uterus from biofilm. Methods: Twenty repeat breeder cows were selected from a large commercial dairy farm near Shiraz, Fars province, southern Iran. Uterine secretion samples were collected before and after uterine lavage with dimethyl sulfoxide (DMSO) 10% solution and periodic acid Schiff (PAS) staining was used to detect bacterial biofilm in uterine samples. After sampling, all cows were treated with two doses of PGF2α and intrauterine infusion of Cefquinome sulphate. Artificial insemination (AI) was performed after that. Results: Bacterial biofilms were found in 12 out of 20 animals (60%) in the first sampling with sterile saline lavage (before DMSO) and in 7 cows (35%) after DMSO lavage. Fourteen cows (70%) became pregnant after AI. This evidence showed the presence of bacterial biofilm in the uterus of dairy cows for the first time. Although non-significant, decrease in biofilm detection after DMSO lavage may suggest the potential ability of mucolytic agent for cleaning the uterus from bacterial biofilm. Also, high pregnancy rate after antibiotic treatment in the present study might be attributed to improved effect of antibiotic following lavage of uterine by DMSO. Conclusions: These findings should be investigated in future researches with more sample size.


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