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Table of Contents
Year : 2017  |  Volume : 6  |  Issue : 6  |  Page : 264-267

Factors affecting in-vivo fertility of crossbred Egyptian - Italian buffalo semen

1 Department of Animal Reproduction and A.I., National Research Center, Dokki, Giza, Egypt
2 General Organization for Veterinary Services, Dokki, Egypt

Date of Submission29-Aug-2017
Date of Decision12-Sep-2017
Date of Acceptance30-Sep-2017
Date of Web Publication01-Nov-2017

Correspondence Address:
KGhM Mahmoud
National Research Center, Dokki, Giza, Postal code 12311, Affiliation I.D. 60014618
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2305-0500.217341

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Objective: To assess the effect of region, season and year of insemination on in-vivo fertility of Italian-Egyptian crossbred buffalo semen. Methods: A total number of 4 799 female buffaloes were inseminated by frozen semen with at least 50% post-thaw motility of Egyptian-Italian crossbred bulls in three localities in Delta, lower Egypt (El-Behira, El-Sharkia and Damietta) during the period of 2013, 2014 and 2015. The pregnancy rate after two months was evaluated during the four seasons. Results: The rate of pregnancy was significantly (P<0.000 1) differ among the three localities. The effect of year of insemination on pregnancy rate was significantly higher during 2014 and 2015 than 2013 in El-Sharkia and El-Behira. But in Damietta, the rate of pregnancy was significantly higher in 2014 than 2013 and 2015. There were no significant differences among seasons in El-Behira and Damietta governorates but there was significant (P<0.05) differences in pregnancy rate in El-Sharkia. It was higher in summer, spring and autumn than in winter. Conclusions: Localities, year of insemination and season of the year have effects on fertility of crossbred Egyptian-Italian buffalo semen.

Keywords: Artificial insemination, Pregnancy rate, Egyptian-Italian crossbred, Buffalo bull

How to cite this article:
El-Sisy G A, Mahmoud K, El-sokary A, Nawito M F, Ahmed Y F. Factors affecting in-vivo fertility of crossbred Egyptian - Italian buffalo semen. Asian Pac J Reprod 2017;6:264-7

How to cite this URL:
El-Sisy G A, Mahmoud K, El-sokary A, Nawito M F, Ahmed Y F. Factors affecting in-vivo fertility of crossbred Egyptian - Italian buffalo semen. Asian Pac J Reprod [serial online] 2017 [cited 2022 Aug 12];6:264-7. Available from: https://www.apjr.net/text.asp?2017/6/6/264/217341

Foundation project: It is supported by National Research Centre (No 1102101).

  1. Introduction Top

Buffaloes are playing essential role in agricultural economy in Egypt. Reproductive efficiency is one of the important aspects of animal breeding. Long calving intervals[1], due to irregular ovarian activity, poor estrus expression and detection[2] are the major constraints in Egyptian buffalo reproduction. The animal productivity can be enhanced by genetic improvement through application of assisted reproductive techniques, such as artificial insemination (AI) and embryo transfer. Unfortunately, keeping buffaloes under the small holder farming system in Egypt constitutes a major challenge against using such technologies. Using of AI with frozen semen exhibited lower fertility in buffalo than in cattle spermatozoa[3],[4],[5],[6],[7],[8],[9],[10]. Moreover, the application of AI in buffaloes faces many difficulties due to low estrous expression and difficult to predict ovulation time[11]. Problems in the field related to AI service include poor awareness of farmer, unskilled technician, irregular supply of nitrogen, long distance, improper detection of estrous and time of insemination[12]. In buffaloes, various causes of lower fertility range from inadequate management in female to poor quality of frozen semen. The overall conception rate through application of artificial insemination technique in cattle and buffalo ranges from 38% to 62%[13] and 29%[12], respectively. To improve productivity of Egyptian buffaloes, there is an essential need to use semen from genetically selected bulls. The Ministry of Agriculture in Egypt accepted the importation of Italian buffalo's semen since 2003 for private farms, as practice needs for improving milk production and reproduction traits[14],[15]. This study aimed to explore the major factors that may affect fertility rates of local breeds of Egyptian buffalo after AI with Egyptian-Italian crossbred bulls under field conditions. The effects of region, years of insemination and season on pregnancy rate were studied.

  2. Materials and methods Top

2.1. Buffalo and geographical location

Total number of 4 799 female buffalo from different governorates (El-Behira, El-Sharkia and Damietta) in north of Delta Egypt were admitted to AI using Egyptian-Italian crossbred bulls frozen semen under the supervision of General Organization for Veterinary Services (GOVS) during the period of 2013, 2014 and 2015. The female buffaloes were inseminated in four seasons of a year by semen with at least 50% post-thaw motility. Weather parameters in studied governorates were illustrated in [Table 1] according to Khalil et al[16].
Table 1: Weather parameters in studied governorates.

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2.2. Bulls and semen

Crossbred Egyptian-Italian buffalo bulls reared in Abbasia semen freezing center, which belongs to GOVS semen source for cryopreservation and insemination program, were used for this study. The cryopreservation extender used in freezing was Bioxcell (IMV, France).

Semen samples were diluted with extender at 37 °C to reach 60 million spermatozoa per milliliter concentrations. After that the semen was cooled to 4 °C slowly for 2 h and equilibrated for 4 h, then put into 0.25 mL straws. After equilibration, the straws were frozen in a vapour above liquid nitrogen by 4 cm for 10 min then were stored in liquid nitrogen.

2.3. In vivo fertility

Female buffalos were artificially inseminated with crossbred Egyptian-Italian semen under the supervision of GOVS. Pregnancy was diagnosed at 60-70 d after insemination by rectal palpation. The pregnancy rates of crossbreed Italian-Egyptian buffalo were recorded by our team in Damietta governorate. Data from other governorates were obtained from AI records of GOVS.

2.4. Statistical analysis

Data were statistically analyzed by ANOVA using SPSS version 16.0. Means of pregnancy rates for different localities, years and seasons were compared by Duncan's test. Differences at P<0.05 were considered to be significant.

  3. Results Top

The pregnancy rates in three different localities of El-Behira, El- Sharkia and Damietta were reported in [Table 2]. The results showed that the rate of pregnancy was significantly (P<0.000 1) different among three localities. It was higher in El-Behira and El-Sharkia than Damietta governorate.
Table 2: Effect of localities on pregnancy rate of crossbred (Egyptian-Italian) buffalo bulls.

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The effect of year of insemination on pregnancy rate was illustrated in [Table 3]. The pregnancy rate was recorded during 2013, 2014 and 2015. In El-Behira, the pregnancy rate was significantly higher during 2014 (P<0.01) and 2015 (P<0.05) than 2013. Also, in El- Sharkia, it was significantly increased in 2014 (P<0.001) and 2015 (P<0.000 1) compared with 2013. But in Damietta, the rate of pregnancy was significantly higher in 2014 (P<0.01) than 2013 and 2015.
Table 3: Effect of year of insemination on pregnancy rate of crossbred (Egyptian-Italian) buffalo bull.

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The effect of season on pregnancy rate of Egyptian-Italian crossbred buffalo was presented in [Table 4]. There were no significant differences among seasons in El-Behira and Damietta governorates. But, the difference in pregnancy rate was significant in El-Sharkia (P<0.05). It was higher in summer, spring and autumn than winter season.
Table 4: Effect of season on pregnancy rate of crossbred (Egyptian-Italian) buffalo bulls.

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  4. Discussion Top

Genetic improvement through crossbreeding and better management should go hand-in-hand to improve the animal reproduction. In Egypt, genetic improvement of the indigenous buffalos focusing on cross breeding has been practiced by GOVS since 2012. The success of crossbreeding programmes in particular needs to be monitored regularly by assessing the in vivo fertility. However, information about evaluation of crossbred buffalo bull is limited particularly in Egypt[17]. The fertility rate is the most important parameter to evaluate the frozen-thawed semen quality[18] and affected by sperm quality parameters[19].

Our results showed pregnancy rate of crossbred semen was 65.7% in El-Behira, 62.7% in El-Sharkia and 53.5% in Damietta. The pregnancy rate was higher in El-Behira and El-Sharkia than Damietta governorates. This variation may be attributed to the difference in AI technicians rather than temperature as the three governorates had a minimal temperature fluctuation. In the same time, we have not any information about the age, parity and nutritional status, condition score and health of inseminated animals. These factors affect the conception/pregnancy rate. In this respect, Den Daas[20] documented that non-return rates can be improved by interaction of different factors such as season, management and AI technician. Moreover, Foote[21] reported that many environmental factors affect fertility of inseminating bull while one single bull enhanced fertility by a factor of 4.7[22].

The percentage of pregnancy rate in our study was higher than 45.2% recorded by Barile et al[23] in Italian buffalo inseminated with frozen semen and 55.2% by Mahmoud et al[l7] for Egyptian-Italian crossbred. Al Naib et al[24] reported that bulls with 50% pregnancy rate are categorized as high fertile.

In our study, the effect of year of insemination on pregnancy rate was significantly higher during 2014 and 2015 than 2013 in El- Sharkia and El-Behira. But in Damietta, the rate of pregnancy was significantly higher in 2014 than 2013 and 2015. The variation between the results in different localities may be attributed to differences in technicians who performed insemination. Moreover, various factors affecting fertility as genetic and environment may be put in consideration[25]. The variations in pregnancy rates are attributed to various bull factors[26] as spermatozoa from different bulls have different ability to fertilize buffalo oocytes in vitro[27],[28] due to differences in metabolic activity of sperm cells[29] and lots of semen from the same bull[30].

With respect to the effect of season on pregnancy rate of Egyptian- Italian crossbred buffalo, there were no significant differences among seasons in El-Behira and Damietta governorates. Also, Bhattacharyya et al[31] recorded no significant variation on conception rate among seasons in crossbred Jersy. But in El-Sharkia, pregnancy rate was higher in summer, autumn and spring than winter season. This could be due to the presence of high environmental temperature fluctuation at different season of the year. In this respect, Robinson[32] stated that the cold stress of winter reduce the conception rate in cows. In contrast, Hamam et al[33] in buffalo and Mahmoud and Eashra[34] in cattle found that the quality and percentage of mature oocytes were decreased in hot seasons than in cooled seasons. Moreover, Ono et al[35] in cows stated that the pregnancy rates were significantly decrease (P<0.05) in summer (June-August) comparing with spring (March-May) and winter (December-February).

In conclusion, in vivo fertility was affected by the localities, year of insemination and season and more studies to evaluate other factors affecting the fertility of crossbred are needed.

Conflict of interest statement

We declare that we have no conflict of interest.


The authors acknowledge Abbasia Artificial Insemination Center, for providing semen straws and National Research Centre, for funding the project 1102101.

  References Top

El-Fouly MA, Kotby EA, El Shoby EA. Post-partum ovarian activity in suckled and milked buffaloes. Theriogenology 1976; 5: 69-81.  Back to cited text no. 1
Khattab RM, Aboul-Ela MB, Barkawi AH, Abdelaal AE. Physiological causes influencing open period in Egyptian buffaloes of hot season calvers. Buffalo J 1990; 6: 153-160.  Back to cited text no. 2
Kakar SS, Anand SR. Changes in adenosine 5ø-triphosphate, adenylate energy charge and adenosine 3ø5ø-cyclic monophosphate during the freezing of buffalo semen. J Reprod Fert 1981; 62, 543-548.  Back to cited text no. 3
Muer SK, Roy SB, Mohan G, Dhoble RL. Cryogenic changes in seminal protein of cattle and buffalo. Theriogenology 1988; 30: 1005-1010.  Back to cited text no. 4
Raizada BC, Sattar A, Pandey MD. A comparative study of freezing buffalo semen in two dilutors. In: Acharya RM, Lokeshwar RR, Kumar AT (eds). Proceedings of 2nd World Buffalo Cong, New Delhi, India. Roma, Italy: International Buffalo Federation; 1990, p. 66-74.  Back to cited text no. 5
Singh M, Pant HC. Effect of post-thaw incubation on semen quality of buffalo bulls - comparison with cattle. Buffalo Bull 2000; 19: 51-54.  Back to cited text no. 6
Andrabi SMH, Ahmad N, Abbas A, Anzar M. Effect of two different antibiotic combinations on fertility of frozen buffalo and Sahiwal bull semen. Pak Vet J 2001; 21: 166-169.  Back to cited text no. 7
Ahmad Z, Anzar M, Shahab M, Ahmad N, Andrabi SMH. Sephadex and sephadex ion-exchange filtration improves the quality and freezability of low-grade buffalo semen ejaculates. Theriogenology 2003; 59: 1189-1202.  Back to cited text no. 8
Senatore EM, Verberckmoes S, Pascale M, Presicce GA. A deep utero-tubal semen deposition in Mediterranean Italian buffaloes using a new artificial insemination device. Reprod Fertil Dev 2004; 16: 133.  Back to cited text no. 9
Kumaresan A, Ansari MR, Abhishek G. Modulation of post-thaw sperm functions with oviductal proteins in buffaloes. Anim Reprod Sci 2005; 90: 73-84.  Back to cited text no. 10
Baruselli, PS. Basic requirement for artificial insemination and embryo transfer in buffaloes. Buffalo J 1994; 2(suppl): 53-60.  Back to cited text no. 11
Anzar M, Farooq U, Mirza MA, Shahab M, Ahmad N. Factors affecting the efficiency of artificial insemination in cattle and buffalo in Punjab, Pakistan. Pakistan Vet J 2003; 23(3): 106-113.  Back to cited text no. 12
Saji MA. Artificial insemination in buffalo. Proceeding Natural Workshop on proceeding and use of buffalo semen. Islamabad: Natural Agriculture Research Center; 1984, p . 27-28.  Back to cited text no. 13
Fooda TA, Elbeltagy AR, Hassan RL, SetEl-habaeib SA. Assessment of Egyptian buffaloes crossing with Pakistani and Italian buffaloes for some production traits. J Am Sci 2011; 7(1): 269-276.  Back to cited text no. 14
Fooda TA, Elbeltagy AR, Laila Hassan R, SetEl-habaeib SA. Evaluated of Egyptian buffaloes crossing with Italian buffaloes for reproductive traits. J Am Sci 2011; 7(7): 209-213.  Back to cited text no. 15
Khalil F, Ouda S, Osman NA, Ghamis A. Determination of agro-climatic zones in Egypt using a robust statistical procedure. Fifteenth International Water Technology Conference, IWTC-15 2011, Alexandria, Egypt; 2011, p. 2101-2110.  Back to cited text no. 16
Mahmoud K Gh M, El-Sisy GA, Mohamed AA, El-Sokary AAE, Nawito M. Comparison of semen freezability and in-vivo fertility of Egyptian and Egyptian-Italian crossbred buffalo bulls. Global Vet 2016; 16(5): 472-475.  Back to cited text no. 17
Vale WG. News on reproductive biotechnology in males. Procceeding 5th World Buffalo Congress Caserta, Italy; 1997, p. 103-123.  Back to cited text no. 18
Soderquist L, Janson K, Larsson K, Einarsson S. Sperm morphology and fertility in AI bulls. J Vet Med 1991; 38: 534-543.  Back to cited text no. 19
Den Daas N. Laboratory assessment of semen characteristics. Anim Reprod Sci 1992; 28: 87-94.  Back to cited text no. 20
Foote RH. Fertility estimation: a review of past experience and future prospects. Anim Reprod Sci 2003; 75: 119-139.  Back to cited text no. 21
López-Gatius F, Santolaria P, Mundet I, Yániz JL. Walking activity at estrus and subsequent fertility in dairy cows. Theriogenology 2005; 63: 1419-1429.  Back to cited text no. 22
Barile V, Galasso A, Pacelli C, Francello M, Cigliano A, Penna L, et al. Conception rate in synchronized and artificially inseminated buffalo cow in different season under field conditions. Proceeding of the A.S.P.A. XIII Congress Piacenza; 1999, p. 262-264.  Back to cited text no. 23
Al Naib A, Hanarahan JP, Lonergan P, Fair S. In vitro assessment of sperm from bulls of high and low fertility. Theriogenology 2011; 76: 161-167.  Back to cited text no. 24
López-Gatius, F. Approaches to increase reproductive efficiency in artificially inseminated dairy cows. Anim Reprod 2013; 10(3): 143-147.  Back to cited text no. 25
Haugan T, Gröhn YT, Kommisrud E, Ropstad E, Reksen O. Effects of sperm concentration at semen collection and storage period of frozen semen on dairy cow conception. Anim Reprod Sci 2006; 97: 1-11.  Back to cited text no. 26
Mahmoud K Gh M, Ziada Maha S, Scholkamy TH. Freezability of buffalo semen in relation to its in-vitro fertilizing capacity and chromosomal picture. J Egypt Vet Med Assoc 2004; 64: 239-251.  Back to cited text no. 27
Abdel Dayem AMH, Mahmoud K Gh M, Nawito MF, Ayoub MM, Scholkamy TH. Fertility evaluation in Egyptian buffalo bulls using zona pellucida binding and in vitro fertilization assays. Livestock Sci 2009; 122: 193-198.  Back to cited text no. 28
Brackett BG, Oliphant G.. Capacitation of rabbit spermatozoa in vitro. Biol Reprod 2009; 12: 260-274.  Back to cited text no. 29
Otoi T, Tachikawa S, Kondo S, Suzuki T. Effect of different lots of semen from the same bull on in vitro development of bovine oocytes fertilized in vitro. Theriogenology 1993; 39: 713-718.  Back to cited text no. 30
Bhattacharyya HK, Fazili MR, Khan I, Bhat FA. Fertility status of artificially inseminated crossbred cows of Kashmir valley. Asian J Anim Sci 2010; 4(2): 56-63.  Back to cited text no. 31
Robinson V. Effect of cold stress on the submission and conception rates of New Zealand dairy cows. Master thesis. New Zealand: Massey University; 2015.  Back to cited text no. 32
Hamam AM, Mahmoud K Gh M , Nawito MF, Seida AA, Nawar SMA. Effect of the seasonal changes on recovery, quality and maturation of buffalo oocytes in vitro. Egypt J Vet Sci 2001; 35: 123-133.  Back to cited text no. 33
Mahmoud K Gh M, Eashra NT. Meiotic chromosomes of cattle oocytes in relation to seasonal variation. Egypt J Vet Sci 2004; 38: 1-9.  Back to cited text no. 34
Ono T, Isobe T, Morita Y, Do LTK, Tanihara F, Taniguchi M, et al. Effects of parity and season on pregnancy rates after the transfer of embryos to repeat-breeder Japanese Black beef cattle. Arch Anim Breed 2016; 59: 45-49.  Back to cited text no. 35


  [Table 1], [Table 2], [Table 3], [Table 4]


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