• Users Online: 267
  • Print this page
  • Email this page
ORIGINAL ARTICLE
Year : 2020  |  Volume : 9  |  Issue : 2  |  Page : 70-76

Effect of short-term gavage of ethanolic extract of cogon grass (Imperata cylindrica L) root on the ovarian activity and estrus behavior of female mice


1 Laboratory of Animal Reproduction and Artificial Insemination, Department of Animal Production, Faculty of Animal Husbandry; Central Laboratory, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km.21 Jatinangor Sumedang, West Java 45363, Indonesia
2 Laboratory of Embryology, Department of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, IPB University, Jl. Agatis Dramaga Bogor 16680, West Java, Indonesia
3 Central Laboratory; Department of Biomedical Science Faculty of Medicine, Universitas Padjadjaran, Jl. Raya Bandung Sumedang Km.21 Jatinangor Sumedang, West Java 45363, Indonesia
4 Department of Biomedical Science Faculty of Medicine, Universitas Padjadjaran, Jl. Raya Bandung Sumedang Km.21 Jatinangor Sumedang, West Java 45363, Indonesia
5 Department of Obstetrics and Gynaecology, Faculty of Medicine, Universitas Padjadjaran, Hasan Sadikin Hospital, Jl. Pasteur No. 38 Bandung, West Jawa Barat 40161, Indonesia
6 Study Program of Undergraduate Medicine, Faculty of Medicine, Universitas Padjadjaran, Jl. Raya Bandung Sumedang Km.21 Jatinangor Sumedang, West Java 45363, Indonesia
7 Department of Obstetrics and Gynaecology, Faculty of Medicine, Udayana University, Jl. PB Sudirman, Denpasar, Bali, Indonesia

Correspondence Address:
Jaqueline Sudiman
Department of Obstetrics and Gynaecology, Faculty of Medicine, Udayana University, Jl. PB Sudirman, Denpasar, Bali
Indonesia
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2305-0500.281076

Rights and Permissions

Objective: To assess the effect of short-term gavage of ethanolic extract of Imperata cylindrica L root on the ovarian activity and estrus behavior of female mice. Methods: Eighteen virgin female ddY mice, 8 to 10 weeks of age, weighing 22–25 g with regular estrus cycle, were divided into three groups. Group 1 received 0.5% carboxymethylcellulose, whereas Groups 2 and 3 received the ethanolic extract of Imperata cylindrica L root at 90 and 115 mg/kg body weight (b.w.) per day by gavage for 20 days, respectively. All of the groups were checked before 9 a.m. daily for vaginal cytology to determine the estrus phase. On day 21, the mice were sacrificed to collect serum samples to quantify the concentrations of reproductive hormones using enzyme-linked immunosorbent assay and to determine changes in the reproductive organs based on their reproductive organ weight, histomorphology, and histomorphometry of ovarium and uterus. Results: The reproductive organ weight in the treatment groups was similar compared with that in the control group. The 90 mg/kg b.w. treatment group showed an increase in corpus luteum number when compared with the control group, with few degenerated follicles and diminished oocytes. Moreover, the 115 mg/kg b.w. treatment group showed fewer primordial and primary follicles and an increase in corpus luteum number and a prolonged diestrus phase compared to the control and 90 mg/kg b.w. treatment groups. The histomorphology examination of the uterus showed that the thickness of myometrium and epithelium in the treated animals was similar to the control group. In addition, there was a significant decrease in follicle-stimulating hormone level in the 115 mg/kg b.w. treatment group (P<0.05). Conclusions: Short-term gavage of ethanolic extract of Imperata cylindrica L root reduces the follicle-stimulating hormone serum level and folliculogenesis.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1973    
    Printed97    
    Emailed0    
    PDF Downloaded245    
    Comments [Add]    
    Cited by others 1    

Recommend this journal