• Users Online: 1366
  • Print this page
  • Email this page
ORIGINAL ARTICLE
Year : 2020  |  Volume : 9  |  Issue : 2  |  Page : 77-82

Effect of aqueous seed extract of Mucuna pruriens on arsenic-induced testicular toxicity in mice


1 Department of Physiology, Melaka Manipal Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India
2 Department of Pharmacology, RAK College of Medical Sciences, RAK Medical and Health Sciences University, Ras Al Khaimah, United Arab Emirates

Correspondence Address:
Preethi Concessao
Department of Physiology, Melaka Manipal Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, 576104
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2305-0500.281077

Rights and Permissions

Objective: To investigate the protective effects of Mucuna (M.) pruriens against arsenic-induced testicular impairment in albino mice. Methods: Thirty-six male albino mice were divided into six groups, with 6 mice in each group. Group 1 received drinking water as the normal control. Groups 2 to 6 received sodium arsenite (40 mg/L) in drinking water. Group 3 received sodium arsenite (40 mg/L) and 300 mg/kg body weight (b.w.) of N-acetylcysteine. Group 4 received sodium arsenite (40 mg/L) and 500 mg/kg b.w. of M. pruriens. Group 5 received sodium arsenite (40 mg/L) and 1 000 mg/kg b.w. of M. pruriens. Group 6 received sodium arsenite (40 mg/L) and 2 000 mg/kg b.w. of M. pruriens. N-acetylcysteine and M. pruriens were administered orally once a day. Animals were subjected to the above treatments for 45 days. Animals were sacrificed with overdose of ketamine 24 h following drug administration. The testis was used for biochemical estimations (lipid peroxidation and glutathione), and the epididymis was used to determine the sperm count and morphology. Results: Sodium arsenite significantly decreased (P <0.01) the sperm count and glutathione levels of the testis. It significantly increased (P <0.01) the abnormal architecture of the spermatozoa and lipid peroxidation levels. Treatment with M. pruriens significantly increased the sperm count and the glutathione levels [500 mg/kg b.w. and 1 000 mg/kg b.w. (P <0.01)]. A significant decrease in sperm abnormality and lipid peroxidation levels [500 mg/kg b.w. and 1 000 mg/kg b.w. (P <0.01)] was also observed. Conclusions: The perturbed sperm parameters and antioxidant levels of the arsenic insulted testis are attenuated by 500 mg/kg b.w. and 1 000 mg/kg b.w. M. pruriens.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1853    
    Printed98    
    Emailed0    
    PDF Downloaded208    
    Comments [Add]    
    Cited by others 1    

Recommend this journal