• Users Online: 282
  • Print this page
  • Email this page
ORIGINAL ARTICLE
Year : 2021  |  Volume : 10  |  Issue : 2  |  Page : 90-96

Evaluation of pig oocyte in vitro maturation and fertilization using three gonadotropin-based hormonal compounds


1 Autonomous University of Mexico State, Amecameca, Mexico
2 Faculty of Sciences, National Autonomous University of Mexico, Mexico
3 National Autonomous University of Mexico, FES Cuautitlan: Km 2.5 Carretera Cuautitlan-Teoloyucan, 54714 Cuautitlan Izcalli, Estado de Mexico, Mexico

Correspondence Address:
Alfredo Medrano
National Autonomous University of Mexico, FES Cuautitlan: Km 2.5 Carretera Cuautitlan-Teoloyucan, 54714 Cuautitlan Izcalli, Estado de Mexico
Mexico
Login to access the Email id

Source of Support: This research was completed through several grants from Universidad Nacional Autonoma de Mexico (PAPIIT IN220419, IN219620, and PIAPI 1810, 2030), Conflict of Interest: None


DOI: 10.4103/2305-0500.311618

Rights and Permissions

Objective: To evaluate the effect of human chorionic gonadotropins (hCG) and equine chorionic gonadotropins (eCG) on in vitro gilt oocyte maturation and embryonic development, using frozen semen for fertilization. Methods: Two independent experiments (6 replicates each) were carried out to evaluate gilt oocyte maturation, and fertilization and embryonic development by using ovaries from a local abattoir. Totally, 712 oocytes were randomly distributed in four-well dishes to receive Novormon (eCG 5.0 IU), PG600 (eCG 5.0 IU and hCG 2.5 IU), Chorulon (hCG 5.0 IU), or no hormones. Oocytes were incubated with 5% CO2, 95% air and saturation humidity at 39 °C for 44 h. Maturation of the oocytes to metaphase II was assessed by using the aceto-orcein technique. In addition, 741 oocytes were used and randomly distributed in four-well dishes, and then oocyte maturation was carried out as mentioned, but matured oocytes were washed and placed in fertilization medium with frozen-thawed sperm. Gametes were co-incubated for 7 h, and then washed and placed in development medium, and incubated for further 7 days, at which time embryonic development was evaluated. Fertilization and embryo development media were not supplemented with the studied hormones. Results: Novormon (eCG) and PG600 (eCG+hCG) treatments significantly improved the percentages of metaphase II oocytes compared to the control group (P<0.05). Furthermore, a significant increase was also observed in the young blastocyst stage between the control group and the PG600 treatment group (P<0.05). Conclusions: Hormonal products Novormon (eCG) and PG600 (eCG+hCG) can obtain the highest percentages of in vitro maturation in gilt oocytes; however, this effect is not transferred to fertilization rates.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed253    
    Printed0    
    Emailed0    
    PDF Downloaded43    
    Comments [Add]    

Recommend this journal