Adansonia digitata aqueous leaf extract ameliorates dexamethasone-induced testicular injury in male Wistar rats
Joseph Babatunde Dare1, Abdulfatai Olakunle Ojewale2, Olugbemi Tope Olaniyan3, John Adakole Adole4, Gloria Enevwo Okotie5, Christopher Oloruntoba Akintayo6, Ayobami Dare7
1 Department of Anatomy, Osun State University, College of Health Sciences, Osogbo, Osun; Department of Anatomy, Bingham University Karu, Nasarawa State, Nigeria
2 Department of Anatomy, Faculty of Basic Medical Sciences, Lagos State University, College of Medicine, Ikeja, Lagos, Nigeria
3 Laboratory for Reproductive Biology and Developmental Programming, Department of Physiology, Edo University, Uzairue, Iyamho, Edo State, Nigeria
4 Department of Anatomy, Bingham University Karu, Nasarawa State, Nigeria
5 Department of Physiology, University of Ibadan, Ibadan, Nigeria
6 Department of Physiology, Afe Babalola University, Ado-Ekiti, Ekiti, Nigeria
7 Department of Physiology, Faculty of Basic Medical Sciences, Bingham University, Karu, Nasarawa State, Nigeria
Abdulfatai Olakunle Ojewale
Department of Anatomy, Faculty of Basic Medical Sciences, Lagos State University, College of Medicine, Ikeja, Lagos
Source of Support: None, Conflict of Interest: None
Objective: To evaluate the effects of aqueous leaf extract of Adansonia (A.) digitata L on dexamethasone-induced testicular damage in male Wistar rats.
Methods: Twenty adult male Wistar rats weighing 170-190 g were divided into four groups. Group I received 0.5 mL of phosphate buffer orally for 28 days and served as the normal control group; group II received 10 mg/kg of dexamethasone (a synthetic glucocorticoid) intraperitoneally for 7 days and 0.5 mL of phosphate buffer orally for 21 days, group III received 10 mg/kg of dexamethasone for 7 days and 800 mg/kg of A. digitata extract orally for 21 days; group IV received 10 mg/kg of dexamethasone for 7 days and 300 mg/kg of vitamin-E orally for 21 days. Dexamethasone was administered intra-peritoneally for 7 days and all administration lasted for 28 days. The rats were sacrificed by anesthesia with diethyl ether and the testes of each animal were harvested. The testis was homogenized in 0.25 M sucrose at 4 °C for biochemical and histological analyses.
Results: Administration of dexamethasone significantly decreased body weight, glucose-6-phosphate dehydrogenase (G6PDH), lactate dehydrogenase (LDH), superoxide dismutase (SOD), and glutathione peroxidase (GPx) (P<0.05), and significantly increased malondialdehyde (MDA) activities (P<0.05). The degeneration in the population of spermatogonia and vacuolation and abnormal widening of the interstitial spaces were observed in the rats treated with dexamethasone. However, administration of A. digitata significantly increased SOD, GPx, G6PDH, and LDH levels, significantly decreased MDA activities and improved the histoarchitecture of the testis (P<0.05).
Conclusions: A. digitata may have an ameliorative effect on dexamethasone-induced testicular damage in Wistar rats because of its anti-inflammatory and antioxidant properties.