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ORIGINAL ARTICLE
Year : 2021  |  Volume : 10  |  Issue : 3  |  Page : 137-144

Vitamin E supplementation may negatively affect preimplantation development and mitochondrial ultrastructure of vitrified murine embryos


1 Maternofetal and Embryo Research Group, Faculty of Medicine, Universiti Teknologi MARA, Selangor Branch, Sungai Buloh Campus, 47000 Sungai Buloh, Selangor; Faculty of Applied Sciences, Universiti Teknologi MARA, Perak Branch, Tapah Campus, 35400 Tapah Road, Perak, Malaysia
2 Maternofetal and Embryo Research Group, Faculty of Medicine, Universiti Teknologi MARA, Selangor Branch, Sungai Buloh Campus, 47000 Sungai Buloh, Selangor, Malaysia
3 Maternofetal and Embryo Research Group, Faculty of Medicine, Universiti Teknologi MARA, Selangor Branch, Sungai Buloh Campus, 47000 Sungai Buloh; Faculty of Health Sciences, Universiti Teknologi MARA, Selangor Branch, Puncak Alam Campus, Bandar Puncak Alam, 42300 Kuala Selangor, Selangor, Malaysia
4 Faculty of Pharmacy, Universiti Teknologi MARA, Selangor Branch, Puncak Alam Campus, Bandar Puncak Alam, 42300 Kuala Selangor, Selangor, Malaysia

Correspondence Address:
Nor-Ashikin Mohamed Noor Khan
Maternofetal and Embryo Research Group, Faculty of Medicine, Universiti Teknologi MARA, Selangor Branch, Sungai Buloh Campus, 47000 Sungai Buloh, Selangor
Malaysia
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2305-0500.316626

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Objective: To observe the effects of vitamin E on post-vitrification preimplantation development, gross morphology as well as mitochondrial distribution and ultrastructure. Methods: Twenty-four female C57BL/6NTac mice, aged 12-16 weeks, were randomly divided into four groups. Group A did not receive any treatment and served as the control group. Group B was treated with corn oil stripped of tocopherols and served as the vehicle group. Group C was treated with 60 mg/kg body weight of tocotrienol-rich-fraction with corn oil stripped of tocopherols. Group D was treated with 60 mg/kg body weight of alpha-tocopherol with corn oil stripped of tocopherols. All treatments were administered orally for 7 consecutive days. After superovulation and mating with fertile males, 2-cell stage embryos were harvested for vitrification. Post vitrification development in vitro, gross morphology and ultrastructure were compared between groups. Results: The number of 2 and 8-cell embryo, and blastocysts in the treatment and control groups were not significantly different (P>0.05). Following vitrification, all 2-cell embryos had equal-sized blastomeres and intact zona pellucida. Mitochondrial aggregation toward the perinuclear region was seen in all of the treatment groups. Both groups C and D had vacuolated mitochondria, which was reflected in the trend of preimplantation development reduction. Conclusions: Vitamin E supplementation of 60 mg/kg body weight does not improve the viability of healthy embryos according to this study. As a result, the most effective dose of vitamin E supplementation may be determined by the initial quality of the embryos.


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