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ORIGINAL ARTICLE
Year : 2021  |  Volume : 10  |  Issue : 4  |  Page : 187-194

Oral supplementation of selenium improves post-thaw sperm quality in Saanen bucks


1 Department of Animal and Wildlife Sciences, University of Pretoria; Department of Chemical Engineering, Vaal University of Technology, South Africa
2 Department of Chemical Engineering, Vaal University of Technology, South Africa

Correspondence Address:
Kambulu Lukusa
Department of Animal and Wildlife Sciences, University of Pretoria; Department of Chemical Engineering, Vaal University of Technology
South Africa
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Source of Support: This study was supported by the National Research Foundation (Project 86181: N00460) and University of Pretoria under UP Postgraduate Research Support Bursary 2014-2018 (K Lukusa-23347971), Conflict of Interest: None


DOI: 10.4103/2305-0500.321126

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Objective: To determine the effect of oral selenium supplementation and semen collection methods on various post thaw semen quality parameters in Saanen bucks. Methods: Sixteen healthy bucks were divided into two equal groups (n=8 each). The treatment group received selenium at 10-day intervals for three months. Sperm kinematic parameters, morphological parameters, mitochondrial membrane potential, plasma membrane functionality, and sperm DNA integrity were evaluated weekly pre and post-cryopreservation. Results: The mean percentages of the morphological abnormalities were significantly lower in the selenium-supplemented samples when semen was collected by using artificial vagina method (P<0.05). Proximal droplet defects were significantly lower in the selenium supplementation group when semen was collected by electro-ejaculation (P<0.05). Post-thaw sperm parameters such as total motility and progressive motility were significantly higher when semen was obtained by artificial vagina in the selenium-supplemented bucks compared to the electro-ejaculation and the control groups (P<0.05). The sperm kinematic parameters such as curvilinear velocity, average path velocity, and amplitude of lateral head displacement were significantly higher when semen was collected by artificial vagina in the selenium-treated bucks (P<0.05). The percentages of sperm with intact and functional plasma membrane and functional mitochondria were significantly higher in the selenium-supplemented samples collected with artificial vagina compared to the electro-ejaculation method and the control groups (P<0.05). In vitro fertilizing potential was significantly higher in the selenium-supplemented samples collected with artificial vagina compared to the electro-ejaculation method and the control groups, respectively (P<0.05). Conclusions: Oral supplementation of selenium and artificial vagina semen collection improve post thaw sperm quality parameters of Saanen buck.


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