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ORIGINAL ARTICLE
Year : 2021  |  Volume : 10  |  Issue : 5  |  Page : 232-238

In-vitro effect of Peganum harmala total alkaloids on spermatozoa quality and oxidative stress of epididymal ram semen


Department of Biological Sciences of the Environment, Faculty of Nature and Life Sciences, Université de Bejaia, 06000 Bejaia, Algeria

Correspondence Address:
Abdelhanine Ayad
Department of Biological Sciences of the Environment, Faculty of Nature and Life Sciences, Université de Bejaia, 06000 Bejaia
Algeria
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2305-0500.326721

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Objective: To determine the in-vitro effect of the total alkaloid extract of Peganum (P.) harmala seeds on ram epididymal sperm. Methods: Semen was divided into six groups according to the following concentrations of the P. harmala total alkaloids: 1, 5, 10, 50, and 100 μg/mL, and the control group. The samples were incubated at ambient temperature (21 °C-24 °C) for 24 h, and analyzed in terms of motility, membrane integrity, and oxidative status. Results: The sperm kinematic parameters, i.e. straight-line velocity, curvilinear velocity, average path velocity, were significantly higher when treated with P. harmala at concentrations ranging from 1 to 10 μg/mL compared to the control group (P<0.05). In addtion, the highest amplitude of the lateral head displacement value was found in the groups treated with concentrations 1 and 5 μg/mL of P. harmala compared to the control group (P<0.05). Total and progressive motilities showed that the extracts at 1, 5, and 10 μg/mL exhibited a high percentage after 24 h of incubation. The effect of P. harmala extracts on the membrane integrity of ram epididymal sperm was concentration-dependent and significantly different compared to the control group (P<0.05). Non-significantly lower lipid peroxidation levels were observed after 24 h of incubation of ram epididymal sperm treated with concentrations 1, 5, and 10 μg/mL of P. harmala extracts compared to the control group (P>0.05). Conclusions: Low concentrations (1-10 μg/mL) of P. harmala extracts stimulate sperm motility, preserve membrane integrity and protect ram spermatozoa from lipid peroxidation.


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