• Users Online: 166
  • Print this page
  • Email this page
ORIGINAL ARTICLE
Year : 2022  |  Volume : 11  |  Issue : 2  |  Page : 93-99

Protective effect of Scrophularia striata combined with trehalose and cysteine added to diluents on cryopreservd goat epididymal sperm


Laboratory of Reproduction Biology, Department of Animal Sciences, Faculty of Agriculture, University of Kurdistan, Sanandaj, Kurdistan, Iran

Correspondence Address:
Abbas Farshad
Laboratory of Reproduction Biology, Department of Animal Sciences, Faculty of Agriculture, University of Kurdistan, Sanandaj, Kurdistan
Iran
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2305-0500.341116

Rights and Permissions

Objective: To evaluate antioxidant effects of Scrophularia (S.) striata ethanol extract, trehalose and cysteine added to diluents on cryopreserved goat epididymal sperms. Methods: Motility and standard motion parameters of sperm were assessed by using computer assisted sperm motility analysis system. Sperm viability was evaluated by eosin-nigrosin staining method. Hypo-osmotic swelling test was used to evaluate membrane health. Thiobarbituric acid testing was used to measure malondialdehyde (MDA) concentrations. To assess DNA fragmentation, sperm chromatin dispersion test was used. In Experiment 1, treatments consisting of basal Tris diluent supplemented with 25, 50 or 100 μg/mL of S. striata ethanol extract gave the best concentration to the freezing diluents. Experiment 2 was carried out to compare the best concentration of S. striata ethanol extract (50 μg/mL) resulting from the first experiment with 150 mM trehalose and/or 5 mM cysteine alone or in combination. Results: S. striata ethanol extract (50 μg/mL) significantly increased sperm viability, motility and progressive motility and at the same time decreased MDA concentration and DNA fragmentation compared to other treatments (P<0.05). In addition, all treatment groups resulted in viability, membrane health, total motility, progressive motility, curvilinear velocity, straight-line velocity higher and MDA lower compared to the control group (P<0.05). Acrosome integrity was significantly higher in 50 μg/mL of S. striata ethanol extract combined with cysteine, trehalose, or cysteine+trehalose groups than those in the control, trehalose, cysteine, and 50 μg/mL of S. striata ethanol extract groups (P<0.05). Regarding DNA, extenders supplemented with 50 μg/mL of S. striata ethanol extract, 50 μg/mL of S. striata ethanol extract+trehalose, and 50 μg/mL of S. striata ethanol extract+trehalose+cysteine were superior to other treatments. Conclusions: Adding 50 μg/mL of S. striata ethanol extract alone or in combination with trehalose and cysteine can improve the quality of cryopreserved epididymal sperms of goats.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed373    
    Printed14    
    Emailed0    
    PDF Downloaded82    
    Comments [Add]    

Recommend this journal