• Users Online: 836
  • Print this page
  • Email this page
ORIGINAL ARTICLE
Year : 2023  |  Volume : 12  |  Issue : 1  |  Page : 23-34

A novel herbal combination ameliorates ovarian dysfunction and regulates altered biochemical parameters in rats with letrozole-induced polycystic ovary syndrome


1 Herbal Research Lab (Department of Botany), Ramnarain Ruia Autonomous College, Matunga, Mumbai-400 019, India
2 Therapeutic Drug Monitoring Laboratory, Sion (East), Mumbai-400 022, India

Correspondence Address:
Sunita Shailajan
Herbal Research Lab (Department of Botany), Ramnarain Ruia Autonomous College, Matunga, Mumbai-400 019
India
Login to access the Email id

Source of Support: This study was supported by DST-SERB, Government of India via sanction No. EMR/2017/001106, Conflict of Interest: None


DOI: 10.4103/2305-0500.365229

Rights and Permissions

Objective: To investigate the potential activity of novel herbal combination and novel herbal formulation (syrup) in female Sprague Dawley rats with letrozole-induced polycystic ovary syndrome (PCOS). Methods: Forty-two rats were randomly divided into seven groups with six rats in each group. Group 1 received 0.5% carboxy methylcellulose orally for 37 days and served as the normal control group. Group 2 was orally administered with letrozole of 1 mg/kg for 21 days and served as the PCOS induction group without treatment. Group 3 to 7 were administered with letrozole of 1 mg/kg for 21 days orally to induce PCOS, and then respectively received clomiphene citrate at 1 mg/kg, 100 and 200 mg/kg of novel herbal combination, 200 mg/kg of novel herbal formulation (syrup), and 400 mg/kg of marketed formulation of Pushyanuga churna, once daily for 15 days. Effects of the novel herbal combination and its syrup formulation were evaluated on the hormonal profile, the levels of antioxidants, the lipid profile and on the ovarian morphology, using letrozole-induced PCOS model in rats. Results: Letrozole caused alterations in hormonal levels and lipid levels similar to PCOS and ovarian histology showed presence of ovarian cysts confirming the induction of PCOS in rats. On treatment with the novel herbal combination and its syrup formulation in PCOS-induced rats, the altered hormonal and lipid profiles showed significant recovery to normal levels. Ovarian histology confirmed the restoration of folliculogenesis in the PCOS-induced rats. The treatment with the syrup formulation of novel herbal combination was found to be more effective than novel herbal combination and showed better recovery in various parameters evaluated. The results of the study, however, suggested that treatment with novel herbal combination and its syrup formulation provided minimal protection against oxidative stress caused due to the induction of PCOS. Conclusions: The integrated approach for management of PCOS is to counterbalance the limitations associated with modern therapy. Both the novel herbal combination and the syrup formulation of novel herbal combination show efficacy in the management of PCOS in rats and restore folliculogenesis in the ovary. The syrup formulation of novel herbal combination is most effective in the management of PCOS and shows potential to be developed as an adjuvant therapeutic agent.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed418    
    Printed16    
    Emailed0    
    PDF Downloaded61    
    Comments [Add]    

Recommend this journal