Asian Pacific Journal of Reproduction

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 6  |  Issue : 4  |  Page : 158--163

Effect of hematopoietic stem cell on tumor necrosis factor- α expression, spiral artery remodeling and placental apoptosis in lead-exposed pregnant mice


Widjiati1, Suryo Kuncorojakti1, Hendy Hendarto2, Viski Fitri Hendrawan3, Aulanni'am Aulanni'am3 
1 Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia
2 Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
3 Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, Indonesia

Correspondence Address:
Widjiati
Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya
Indonesia

Objective: To evaluate the effectiveness of hematopoietic stem cell (HSC) on TNF-α expression, spiral artery remodeling, and placental apoptosis. Methods: The study used pregnant mice divided to five groups of treatment: Group 1, without treatment; Group 2, 1/12 LD50 of lead and Aquadest; Group 3, 1/16 LD50 of lead and Aquadest, Group 4, 1/12 LD50 of lead and 1 × 107 HSC; and group 5, 1/16 LD50 of lead and 1 × 107 HSC. Histopathology examination was performed to identify spiral artery remodeling. Immunohistochemistry analysis was conducted to characterize TNF-α expression and the apoptosis index. The data from all five groups were compared using the ANOVA test. Results: TNF-α expression differed significantly among the groups that were not exposed to lead, exposed to lead and exposed to lead after HSC treatment (P<0.01). There was also a significant difference of spiral artery remodeling between groups without lead-exposed, lead exposed and lead exposed followed HSC treatment (P<0.05). In apoptosis index evaluation there was a significant difference between groups without lead-exposed, lead exposed and lead exposed followed HSC treatment, but not in the groups of 1/16 LD50 of lead followed HSC (P>0.05). Conclusions: In lead-exposed pregnant mice, the administration of hematopoietic stem cell may decrease TNF-α expression, spiral artery remodeling, and placental apoptosis index but in the group of 1/16 LD50 of lead and HSC treatment, apoptosis index has no differences with those of control group.


How to cite this article:
Widjiati, Kuncorojakti S, Hendarto H, Hendrawan VF, Aulanni'am A. Effect of hematopoietic stem cell on tumor necrosis factor- α expression, spiral artery remodeling and placental apoptosis in lead-exposed pregnant mice.Asian Pac J Reprod 2017;6:158-163


How to cite this URL:
Widjiati, Kuncorojakti S, Hendarto H, Hendrawan VF, Aulanni'am A. Effect of hematopoietic stem cell on tumor necrosis factor- α expression, spiral artery remodeling and placental apoptosis in lead-exposed pregnant mice. Asian Pac J Reprod [serial online] 2017 [cited 2021 Oct 17 ];6:158-163
Available from: http://www.apjr.net/article.asp?issn=2305-0500;year=2017;volume=6;issue=4;spage=158;epage=163;aulast=Widjiati,;type=0