Asian Pacific Journal of Reproduction

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 9  |  Issue : 1  |  Page : 22--30

Comparative proteomic analysis of mature and immature oocytes in domestic cats


Bongkoch Turathum1, Kulnasan Saikhun2, Sittiruk Roytrakul3, Chinnarat Changsangfa2, Supita Tanasawet4, Morakot Sroyraya5, Yindee Kitiyanant6 
1 Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road; Department of Basic Medical Science, Faculty of Medicine Vajira hospital, Navamindradhiraj University, Bangkok 10300, Thailand
2 Institute of Molecular Biosciences, Mahidol University, NakhonPathom 73170, Thailand
3 National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, Thailand
4 Department of Anatomy, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand
5 Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand
6 Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400; Institute of Molecular Biosciences, Mahidol University, NakhonPathom 73170, Thailand

Correspondence Address:
Bongkoch Turathum
Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road; Department of Basic Medical Science, Faculty of Medicine Vajira hospital, Navamindradhiraj University, Bangkok 10300
Thailand

Objective: To evaluate changes of feline (Felis catus) oocytes proteins during in vitro maturation by using the proteomic approach. Methods: Immature oocytes (germinal vesicle) isolated from female cats were cultured and collected at 0 h and 24 h. After collection, oocytes were investigated into immature (germinal vesicle) and mature (metaphase II) stages. The qualitative profiles of the proteins at the immature and mature stages were determined by one-dimensional electrophoresis and liquid chromatography-mass spectrometry. Results: Our data revealed that following 24 h in vitro maturation the maturation rate (metaphase II stage) was 58.7%. Eighty-one of the 260 proteins analyzed were differentially expressed between the germinal vesicle stage and the metaphase II -arrest stage. Proteomic analysis of germinal vesicle and metaphase II oocytes showed abundant expression of proteins involved in transportation (10%), indicating that this was a major characteristic of germinal vesicle oocytes. Similarly, analysis of the proteome of metaphase II oocytes indicated that cell cycle proteins were overexpressed. Interestingly, proteins involved in DNA repair and apoptosis were only expressed in germinal vesicle oocytes and proteins involved in fertilization were only expressed in metaphase II oocytes. Conclusions: The overexpression of certain proteins in germinal vesicle and metaphase II is necessary for oocyte development and maturation. Our findings provide a valuable resource for further investigations into protein expression in oocytes at different developmental stages.


How to cite this article:
Turathum B, Saikhun K, Roytrakul S, Changsangfa C, Tanasawet S, Sroyraya M, Kitiyanant Y. Comparative proteomic analysis of mature and immature oocytes in domestic cats.Asian Pac J Reprod 2020;9:22-30


How to cite this URL:
Turathum B, Saikhun K, Roytrakul S, Changsangfa C, Tanasawet S, Sroyraya M, Kitiyanant Y. Comparative proteomic analysis of mature and immature oocytes in domestic cats. Asian Pac J Reprod [serial online] 2020 [cited 2021 Oct 17 ];9:22-30
Available from: http://www.apjr.net/article.asp?issn=2305-0500;year=2020;volume=9;issue=1;spage=22;epage=30;aulast=Turathum;type=0