Year : 2022 | Volume
: 11 | Issue : 6 | Page : 276--284
L–arginine alleviates postmenopausal complications in female rats by stimulating ovarian dopamine beta hydroxylase
Fatemeh Lakzaei1, Manizheh Karami1, Mohammadreza Jalali Nadoushan2
1 Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
2 Department of Pathology, Faculty of Medicine, Shahed University, Tehran, Iran
Objective: To evaluate the levels of estrogen, albumin and gonadotropins (luteinizing hormone, follicle-stimulating hormone) as well as the activity of dopamine beta hydroxylase (DAß H) in aged female rats treated with nitric oxide precursor L-arginine and neuronal nitric oxide synthase antagonist L-NAME.
Methods: A total of 224 Wistar rats (36 weeks old, weighing 250 g) based on a random sampling were divided into the control and experimental groups after Pap smear test. The control group received only saline (1 mL/kg) intraperitoneally (i.p.). The experiential groups were treated with L-arginine (5, 25 and 50 mg/kg, i.p.) and L-NAME (5 and 25 mg/kg, i.p.) for 3 to 21 days, once a day. Blood samples were taken from the rats and the levels of estrogen and albumin and gonadotropins in the serum were monitored by enzyme-linked immunosorbent assay kit, and the ovaries were examined immunohistopathologically for DAßH activity.
Results: L-arginine (5 mg/kg) significantly increased estrogen level (P<0.05), which was associated with DAßH activation in the ovaries. L-NAME reduced this effect when administered prior to L-arginine dose. L-arginine caused no significant change in the levels of luteinizing hormone and follicle-stimulating hormone. Except for the lowest dose of L-arginine in the shortest period, albumin levels significantly decreased in other treatments compared to the control group (P<0.05).
Conclusions: L-arginine is likely to reduce postmenopausal problems due to an increased nitric oxide level.
Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
|How to cite this article:|
Lakzaei F, Karami M, Jalali Nadoushan M. L–arginine alleviates postmenopausal complications in female rats by stimulating ovarian dopamine beta hydroxylase.Asian Pac J Reprod 2022;11:276-284
|How to cite this URL:|
Lakzaei F, Karami M, Jalali Nadoushan M. L–arginine alleviates postmenopausal complications in female rats by stimulating ovarian dopamine beta hydroxylase. Asian Pac J Reprod [serial online] 2022 [cited 2022 Nov 26 ];11:276-284
Available from: https://www.apjr.net/article.asp?issn=2305-0500;year=2022;volume=11;issue=6;spage=276;epage=284;aulast=Lakzaei;type=0