Embryo quality and chromosomal abnormality in embryos from couples undergoing assisted reproductive technology using preimplantation genetic screening :Mina Niusha, Seyed Ali Rahmani, Leila Kohan, Ladan Sadeghi, Mohammad Nouri, Hamid Reza Nejabati, Asian Pacific Journal of Reproduction

ORIGINAL ARTICLE
Year : 2023 | Volume : 12 | Issue : 1 | Page : 16--22

Embryo quality and chromosomal abnormality in embryos from couples undergoing assisted reproductive technology using preimplantation genetic screening

Mina Niusha¹, Seyed Ali Rahmani², Leila Kohan¹, Ladan Sadeghi¹, Mohammad Nouri³, Hamid Reza Nejabati⁴
¹ Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, Iran
² Department of Medical Genetics, Tabriz University of Medical Sciences, Tabriz, Iran
³ Department of Reproductive Biology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences Tabriz, Iran
⁴ Department of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

Correspondence Address:
Seyed Ali Rahmani
Department of Medical Genetics, Tabriz University of Medical Sciences, Tabriz
Iran

Objective: To detect common chromosomal aneuploidy variations in embryos from couples undergoing assisted reproductive technology and preimplantation genetic screening and their possible associations with embryo quality. Methods: In this study, 359 embryos from 62 couples were screened for chromosomes 13, 21, 18, X, and Y by fluorescence insitu hybridization. For biopsy of blastomere, a laser was used to remove a significantly smaller portion of the zona pellucida. One blastomere was gently biopsied by an aspiration pipette through the hole. After biopsy, the embryo was immediately returned to the embryo scope until transfer. Embryo integrity and blastocyst formation were assessed on day 5. Results: Totally, 282 embryos from 62 couples were evaluated. The chromosomes were normal in 199 (70.57%) embryos and abnormal in 83 (29.43%) embryos. There was no significant association between the quality of embryos and numerical chromosomal abnormality (P=0.67). Conclusions: Embryo quality is not significantly correlated with its genetic status. Hence, the quality of embryos determined by morphological parameters is not an appropriate method for choosing embryos without these abnormalities.

How to cite this article:
Niusha M, Rahmani SA, Kohan L, Sadeghi L, Nouri M, Nejabati HR. Embryo quality and chromosomal abnormality in embryos from couples undergoing assisted reproductive technology using preimplantation genetic screening.Asian Pac J Reprod 2023;12:16-22

How to cite this URL:
Niusha M, Rahmani SA, Kohan L, Sadeghi L, Nouri M, Nejabati HR. Embryo quality and chromosomal abnormality in embryos from couples undergoing assisted reproductive technology using preimplantation genetic screening. Asian Pac J Reprod [serial online] 2023 [cited 2023 Mar 25 ];12:16-22
Available from: https://www.apjr.net/article.asp?issn=2305-0500;year=2023;volume=12;issue=1;spage=16;epage=22;aulast=Niusha;type=0