Close
  Indian J Med Microbiol
 

Figure 1: Photomicrograph of the vitrified 2-cell embryos developed to blastocyst stage from the C57BL/6NTac mice from (A) and (E) without treatment (control); (B) and (F) corn oil of stripped tocopherols; (C) and (G) tocotrienol-rich-fraction plus corn oil; (D) and (H) alpha-tocopherol plus corn oil groups, at magnification 200×. Arrows indicate normal morphology and arrowheads indicate abnormal morphology. The zona pellucida of all vitrified embryos are found to be intact [Figure 1]A,[Figure 1]B,[Figure 1]C,[Figure 1]D,[Figure 1]E,[Figure 1]F,[Figure 1]G,[Figure 1]H. The morphology of normal 2-cell embryos across all vitrified groups is similar, showing reestablishment of blastomere shape and size, with a low degree of fragmentation [Figure 1]A,[Figure 1]B,[Figure 1]C,[Figure 1]D. Embryos with abnormal morphology is also detected [Figure 1]A & [Figure 1]C. Embryos with unequal size and shape with fragmented blastomere are observed [Figure 1]B, [Figure 1]C & [Figure 1]D. Normal blastocysts with compacted cell mass, and trophectoderm with many cells lining the blastocoel cavity [Figure 1]E,[Figure 1]F,[Figure 1]G,[Figure 1]H are seen. Degenerated blastocysts are also observed [Figure 1]E, [Figure 1]F & [Figure 1]H.

Figure 1: Photomicrograph of the vitrified 2-cell embryos developed to blastocyst stage from the C57BL/6NTac mice from (A) and (E) without treatment (control); (B) and (F) corn oil of stripped tocopherols; (C) and (G) tocotrienol-rich-fraction plus corn oil; (D) and (H) alpha-tocopherol plus corn oil groups, at magnification 200×. Arrows indicate normal morphology and arrowheads indicate abnormal morphology. The zona pellucida of all vitrified embryos are found to be intact [Figure 1]A,[Figure 1]B,[Figure 1]C,[Figure 1]D,[Figure 1]E,[Figure 1]F,[Figure 1]G,[Figure 1]H. The morphology of normal 2-cell embryos across all vitrified groups is similar, showing reestablishment of blastomere shape and size, with a low degree of fragmentation [Figure 1]A,[Figure 1]B,[Figure 1]C,[Figure 1]D. Embryos with abnormal morphology is also detected [Figure 1]A & [Figure 1]C. Embryos with unequal size and shape with fragmented blastomere are observed [Figure 1]B, [Figure 1]C & [Figure 1]D. Normal blastocysts with compacted cell mass, and trophectoderm with many cells lining the blastocoel cavity [Figure 1]E,[Figure 1]F,[Figure 1]G,[Figure 1]H are seen. Degenerated blastocysts are also observed [Figure 1]E, [Figure 1]F & [Figure 1]H.