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Figure 1: Cumulus cell expansion from buffalo cumulus oocyte complexes after in vitro maturation. The left panel (i) indicates good quality oocytes and right panel (ii) indicates fair quality oocytes in maturation medium. Both qualities of oocytes are cultured at 38.5 °C for 24 h under 5% CO2 in humidified air for in vitro maturation. The expansion of cumulus cells are categorized as grade A (noticeable expansion), grade B (moderate expansion) and grade C (less or zero expansions) which are represented in both images (i, ii) as A, B and C, respectively. Scale bars represent 100 μm. |
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