Figure 2: Influence of L-carnitine on cumulus cell expansion in good and fair quality oocytes. L-carnitine is supplemented with either 0, 0.250, 0.375 or 0.500 mg/mL concentrations of both types of oocytes. Oocytes are cultured at 38.5 °C for 24 h under 5% CO2 in humidified air for in vitro maturation. The cumulus cell expansions are categorized as grade A (noticeable expansion), grade B (moderate expansion) and grade C (less or zero expansions). All the data from the experiments are plotted in IBM SPSS Statistics (Version 22) and examined for normal distribution. Data are shown as the mean±SD from at least four replicated cultures. Bars with different letters (a-e) differ significantly (P<0.05). COCs: cumulus oocyte complexes.