Figure 3: In vitro development of the buffalo embryos from oocytes in vitro matured with supplementation of L-carnitine. Oocytes are cultured at 38.5 °C for 24 h under 5% CO2 in humidified air for in vitro maturation. Then, the oocytes are added in sperm droplets and incubated for 18 h at 38.5 °C with 5% CO2 in air for in vitro fertilization. Then, the zygotes are cultured for 7 days at 38.5 °C with 5% CO2 in air. The day of in vitro fertilization is designated as day 0. Observations are taken on day 2 and day 7 of in vitro culture to examine the cleavage and subsequent development to blastocyst stage of embryos. A: cleaved embryo; B: blastocyst. Scale bars represent 20 μm.